D-Lab — 生体分子動態機能研究室
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本間 道夫
研究員（名古屋大学名誉教授）

本間 道夫

細菌べん毛モーターのエネルギー変換機構

名古屋大学名誉教授。専門は分子微生物学で、細菌べん毛とそのモーターの構造・機能の解明を中心に研究を続けている。

欧文論文

  • 2026Zhang L, Tan J, Duan X, Wang X, Wang T, Yuan K, Homma M, Kojima S, Zhou Y, Zhu Y. (2026) Molecular basis of high-torque transmission of the Vibrio polar flagellar motor. Protein Cell. doi:10.1093/procel/pwag025.
  • 2025Nishikino, T., Takekawa, N., Kishikawa, J.I., Hirose, M., Kojima, S., Homma, M., Kato, T., and Imada, K. (2025) Structural insight into sodium ion pathway in the bacterial flagellar stator from marine Vibrio. Proc Natl Acad Sci U S A. 122: e2415713122.
  • Nishikino, T., Hatano, A., Kojima, S., and Homma, M. (2025) Regulatory Role of a Hydrophobic Core in the FliG C-Terminal Domain in the Rotary Direction of a Flagellar Motor. Biomolecules 15.
  • Ishikawa, F., Takahashi, K., Takaya, A., Tanabe, G., Homma, M., and Uchihashi, T. (2025) Dynamic Oligomerization Processes of Bacillus subtilis ClpP Protease Induced by ADEP1 Studied with High-Speed Atomic Force Microscopy. ACS Omega 10: 7381-7388.
  • 2024Uesaka, K., Inaba, K., Nishioka, N., Kojima, S., Homma, M., and Ihara, K. (2024) Deciphering the genomes of motility-deficient mutants of Vibrio alginolyticus 138-2. PeerJ 12: e17126.
  • Takekawa, N., Nishikino, T., Kishikawa, J.i., Hirose, M., Kinoshita, M., Kojima, S., Minamino, T., Uchihashi, T., Kato, T., Imada, K., and Homma, M. (2024) Structural analysis of S-ring composed of FliFG fusion proteins in marine Vibrio polar flagellar motor. mBio 15: e0126124.
  • Miyamura, Y., Nishikino, T., Koiwa, H., Homma, M., and Kojima, S. (2024) Roles of linker region flanked by transmembrane and peptidoglycan binding region of PomB in energy conversion of the Vibrio flagellar motor. Genes Cells 29: 282-289.
  • Ishikawa, F., Homma, M., Tanabe, G., and Uchihashi, T. (2024) Protein degradation by a component of the chaperonin-linked protease ClpP. Genes Cells 29: 695-709.
  • 2023Terashima, H., Homma, M., and Kojima, S. (2023) Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo. Methods Mol Biol 2646: 71-82.
  • Takahashi, K., Nishikino, T., Kajino, H., Kojima, S., Uchihashi, T., and Homma, M. (2023) Ring formation by Vibrio fusion protein composed of FliF and FliG, MS-ring and C-ring component of bacterial flagellar motor in membrane. Biophys Physicobiol 20: e200028.
  • Nishikino, T., Hijikata, A., Kojima, S., Shirai, T., Kainosho, M., Homma, M., and Miyanoiri, Y. (2023) Changes in the hydrophobic network of the FliG(MC) domain induce rotational switching of the flagellar motor. iScience 26: 107320.
  • Kojima, S., Homma, M., and Kandori, H. (2023) Purification of the Na(+)-Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopy. Methods Mol Biol 2646: 95-107.
  • Fukushima, Y., Homma, M., and Kojima, S. (2023) Interaction of FlhF, SRP-like GTPase with FliF, MS ring component assembling the initial structure of flagella in marine Vibrio. J Biochem 174: 125-130.
  • 2022Nishikino, T., Takekawa, N., Tran, D.P., Kishikawa, J.I., Hirose, M., Onoe, S., Kojima, S., Homma, M., Kitao, A., Kato, T., and Imada, K. (2022) Structure of MotA, a flagellar stator protein, from hyperthermophile. Biochem Biophys Res Commun 631: 78-85.
  • Homma, M., Nishikino, T., and Kojima, S. (2022) Achievements in bacterial flagellar research with focus on Vibrio species. Microbiol Immunol 66: 75-95.
  • Homma, M., Kobayakawa, T., Hao, Y., Nishikino, T., and Kojima, S. (2022) Function and Structure of FlaK, a Master Regulator of the Polar Flagellar Genes in Marine Vibrio. J Bacteriol 204: e0032022.
  • Homma M, Takekawa N, Fujiwara K, Hao Y, Onoue Y, Kojima, S. (2022) Formation of multiple flagella caused by a mutation of the flagellar rotor protein FliM in Vibrio alginolyticus. Genes Cells 27: 568-578. doi: 10.1111/gtc.12975.
  • Homma M, Mizuno A, Hao Y, Kojima S. (2022) Functional analysis of the N-terminal region of Vibrio FlhG, a MinD-type ATPase in flagellar number control. J. Biochem. 172: 99-107. doi:10.1093/jb/mvac047.
  • Homma M and Kojima S. (2022) The periplasmic domain of the ion-conducting stator of bacterial flagella regulates force generation. Front. Microbiol. 13: 869187. doi: 10.3389/fmicb.2022.869187.
  • Terashima H, Hori K, Ihara K, Homma M, Kojima S. (2022) Mutations in the stator protein PomA affect switching of rotational direction in bacterial flagellar motor. Sci Rep. 12(1): 2979. doi: 10.1038/s41598-022-06947-5.
  • Homma M, Kojima S. (2022) Roles of the second messenger c-di-GMP in bacteria: Focusing on the topics of flagellar regulation and Vibrio spp. Genes Cells. doi: 10.1111/gtc.12921.
  • Nishikino T, Sagara Y, Terashima H, Homma M, Kojima S. (2022) Hoop-like role of the cytosolic interface helix in Vibrio PomA, an ion-conducting membrane protein, in the bacterial flagellar motor. J Biochem. mvac001. doi: 10.1093/jb/mvac001.
  • 2021Takekawa N, Nishikino T, Hori K, Kojima S, Imada K, Homma M. (2021) ZomB is essential for chemotaxis of Vibrio alginolyticus by the rotational direction control of the polar flagellar motor. Genes Cells. doi: 10.1111/gtc.12895.
  • Takekawa N, Nishikino T, Yamashita T, Hori K, Onoue Y, Ihara K, Kojima S, Homma M, Imada K. (2021) A slight bending of an α-helix in FliM creates a counterclockwise-locked structure of the flagellar motor in Vibrio. J Biochem. mvab074. doi: 10.1093/jb/mvab074.
  • Homma M, Terashima H, Koiwa H, Kojima S. (2021) Putative Spanner Function of the Vibrio PomB Plug Region in the Stator Rotation Model for Flagellar Motor. J Bacteriol. 203(16): e0015921. doi: 10.1128/JB.00159-21.
  • Takekawa N, Kawamoto A, Sakuma M, Kato T, Kojima S, Kinoshita M, Minamino T, Namba K, Homma M, Imada K. (2021) Two Distinct Conformations in 34 FliF Subunits Generate Three Different Symmetries within the Flagellar MS-Ring. mBio. 12(2): e03199-20. doi: 10.1128/mBio.03199-20.
  • Terashima H, Kojima S, Homma M. (2021) Site-directed crosslinking identifies the stator-rotor interaction surfaces in a hybrid bacterial flagellar motor. J Bacteriol. JB.00016-21. doi: 10.1128/JB.00016-21.
  • Kojima S, Kajino H, Hirano K, Inoue Y, Terashima H, Homma M. (2021) Role of the N- and C-terminal regions of FliF, the MS ring component in Vibrio flagellar basal body. J Bacteriol. JB.00009-21. doi: 10.1128/JB.00009-21.
  • 2020Carroll BL, Nishikino T, Guo W, Zhu S, Kojima S, Homma M, Liu J. (2020) The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching. eLife. 9: e61446. doi: 10.7554/eLife.61446.
  • Takekawa N, Imada K, Homma M. (2020) Structure and Energy-Conversion Mechanism of the Bacterial Na+-Driven Flagellar Motor. Trends Microbiol. 28(9): 719-731. doi: 10.1016/j.tim.2020.03.010.
  • Terashima H, Hirano K, Inoue Y, Tokano T, Kawamoto A, Kato T, Yamaguchi E, Namba K, Uchihashi T, Kojima S, Homma M. (2020) Assembly mechanism of a supramolecular MS-ring complex to initiate bacterial flagellar biogenesis in Vibrio species. J Bacteriol. JB.00236-20. doi: 10.1128/JB.00236-20.
  • Zhuang XY, Guo S, Li Z, Zhao Z, Kojima S, Homma M, Wang P, Lo CJ, Bai F. (2020) Live-cell fluorescence imaging reveals dynamic production and loss of bacterial flagella. Mol Microbiol. 114(2): 279-291. doi: 10.1111/mmi.14511.
  • Kojima S, Terashima H, Homma M. (2020) Regulation of the Single Polar Flagellar Biogenesis. Biomolecules. 10(4): 533. doi: 10.3390/biom10040533.
  • Kojima S, Imura Y, Hirata H, Homma M. (2020) Characterization of the MinD/ParA-type ATPase FlhG in Vibrio alginolyticus and implications for function of its monomeric form. Genes Cells. doi: 10.1111/gtc.12754.
  • Miyata M, Robinson RC, Uyeda TQP, Fukumori Y, Fukushima SI, Haruta S, Homma M, Inaba K, Ito M, Kaito C, Kato K, Kenri T, Kinosita Y, Kojima S, Minamino T, Mori H, Nakamura S, Nakane D, Nakayama K, Nishiyama M, Shibata S, Shimabukuro K, Tamakoshi M, Taoka A, Tashiro Y, Tulum I, Wada H, Wakabayashi KI. (2020) Tree of motility - A proposed history of motility systems in the tree of life. Genes Cells. 25(1): 6-21. doi: 10.1111/gtc.12737.
  • Mengucci F, Dardis C, Mongiardini EJ, Althabegoiti MJ, Partridge JD, Kojima S, Homma M, Quelas JI, Lodeiro AR. (2020) Characterization of FliL Proteins in Bradyrhizobium diazoefficiens: Lateral FliL Supports Swimming Motility, and Subpolar FliL Modulates the Lateral Flagellar System. J Bacteriol. 202(5): e00708-19. doi: 10.1128/JB.00708-19.
  • Nishikino T, Iwatsuki H, Mino T, Kojima S, Homma M. (2020) Characterization of PomA periplasmic loop and sodium ion entering in stator complex of sodium-driven flagellar motor. J. Biochem. 167(4): 389-398. doi: 10.1093/jb/mvz102.
  • Zhu S, Nishikino T, Takekawa N, Terashima H, Kojima S, Imada K, Homma M, Liu J. (2020) In situ structure of the Vibrio polar flagellum reveals distinct outer membrane complex and its specific interaction with the stator. J Bacteriol. 202(4): e00592-19. doi: 10.1128/JB.00592-19.
  • 2019Onoue Y, Iwaki M, Shinobu A, Nishihara Y, Iwatsuki H, Terashima H, Kitao A, Kandori H, Homma M. (2019) Essential ion binding residues for Na+ flow in stator complex of the Vibrio flagellar motor. Sci Rep. 9(1): 11216. doi: 10.1038/s41598-019-46038-6.
  • Sakuma M, Nishikawa S, Inaba S, Nishigaki T, Kojima S, Homma M, Imada K. (2019) Structure of the periplasmic domain of SflA involved in spatial regulation of the flagellar biogenesis of Vibrio reveals a TPR/SLR-like fold. J. Biochem. 166(2): 197-204. doi: 10.1093/jb/mvz027.
  • Mino T, Nishikino T, Iwatsuki H, Kojima S, Homma M. (2019) Effect of sodium ions on conformations of the cytoplasmic loop of the PomA stator protein of Vibrio alginolyticus. J. Biochem. mvz040. doi: 10.1093/jb/mvz040.
  • Takekawa N, Isumi M, Terashima H, Zhu S, Nishino Y, Sakuma M, Kojima S, Homma M, Imada K. (2019) Structure of Vibrio FliL, a New Stomatin-like Protein That Assists the Bacterial Flagellar Motor Function. MBio. 10(2): e00292-19. doi: 10.1128/mBio.00292-19.
  • Kojima S, Yoneda T, Morimoto W, Homma M. (2019) Effect of PlzD, a YcgR homolog of c-di-GMP binding protein, on polar flagellar motility in Vibrio alginolyticus. J Biochem. doi: 10.1093/jb/mvz014.
  • 2018Nishikino T, Hijikata A, Miyanoiri Y, Onoue Y, Kojima S, Shirai T, Homma M. (2018) Rotational direction of flagellar motor from the conformation of FliG middle domain in marine Vibrio. Sci Rep. 8(1): 17793. doi: 10.1038/s41598-018-35902-6.
  • Kondo S, Imura Y, Mizuno A, Homma M, Kojima S. (2018) Biochemical analysis of GTPase FlhF which controls the number and position of flagellar formation in marine Vibrio. Sci Rep. 8(1): 12115. doi: 10.1038/s41598-018-30531-5.
  • Zhu S, Nishikino T, Kojima S, Homma M, Liu J. (2018) The Vibrio H-ring facilitates the outer membrane penetration of polar-sheathed flagellum. J Bacteriol. JB.00387-18. doi: 10.1128/JB.00387-18.
  • Lin TS, Zhu S, Kojima S, Homma M, Lo CJ. (2018) FliL association with flagellar stator in the sodium-driven Vibrio motor characterized by the fluorescent microscopy. Sci Rep. 8(1): 11172. doi: 10.1038/s41598-018-29447-x.
  • Kojima S, Takao M, Almira G, Kawahara I, Sakuma M, Homma M, Kojima C, Imada K. (2018) The Helix Rearrangement in the Periplasmic Domain of the Flagellar Stator B Subunit Activates Peptidoglycan Binding and Ion Influx. Structure 26(4): 590-598.e5. doi: 10.1016/j.str.2018.02.016.
  • Onoue Y, Takekawa N, Nishikino T, Kojima S, Homma M. (2018) The role of conserved charged residues in the bidirectional rotation of the bacterial flagellar motor. Microbiologyopen e00587. doi: 10.1002/mbo3.587.
  • Liew CW, Hynson RM, Ganuelas LA, Shah-Mohammadi N, Duff AP, Kojima S, Homma M, Lee LK. (2018) Solution structure analysis of the periplasmic region of bacterial flagellar motor stators by small angle X-ray scattering. Biochem Biophys Res Commun 495(2): 1614-1619. doi: 10.1016/j.bbrc.2017.11.194.
  • 2017Kondo S, Homma M, Kojima S. (2017) Analysis of the GTPase motif of FlhF in the control of the number and location of polar flagella in Vibrio alginolyticus. Biophys Physicobiol. 14: 173-181. doi: 10.2142/biophysico.14.0_173.
  • Rad MA, Ahmad MR, Nakajima M, Kojima S, Homma M, Fukuda T. (2017) Application of Environmental Scanning Electron Microscope-Nanomanipulation System on Spheroplast Yeast Cells Surface Observation. Scanning 2017: 8393578. doi: 10.1155/2017/8393578.
  • Zhu S, Nishikino T, Hu B, Kojima S, Homma M & Liu J. (2017) Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus. Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1712489114.
  • Miyanoiri Y, Hijikata A, Nishino Y, Gohara M, Onoue Y, Kojima S, Kojima C, Shirai T, Kainosho M, Homma M. (2017) Structural and Functional Analysis of the C-Terminal Region of FliG, an Essential Motor Component of Vibrio Na+-Driven Flagella. Structure doi: 10.1016/j.str.2017.08.010.
  • Inaba S, Nishigaki T, Takekawa N, Kojima S, Homma M. (2017) Localization and domain characterization of the SflA regulator of flagellar formation in Vibrio alginolyticus. Genes Cells 22(7): 619-627. doi: 10.1111/gtc.12501.
  • Onoue Y, Homma M. (2017) Structure of the Sodium-Driven Flagellar Motor in Marine Vibrio. Methods Mol Biol 1593: 253-258. doi: 10.1007/978-1-4939-6927-2_20.
  • Kumar A, Isumi M, Sakuma M, Zhu S, Nishino Y, Onoue Y, Kojima S, Miyanoiri Y, Imada K, Homma M. (2017) Biochemical characterization of the flagellar stator-associated inner membrane protein FliL from Vibrio alginolyticus. J. Biochem. 161(4): 331-337. doi: 10.1093/jb/mvw076.
  • Takekawa N, Kojima S, and Homma M. (2017) Mutational analysis and overproduction effects of MotX, an essential component for motor function of Na+-driven polar flagella of Vibrio. J. Biochem. 161(2): 159-166. doi: 10.1093/jb/mvw061.
  • 2016Onoue Y, Abe-Yoshizumi R, Gohara M, Nishino Y, Kobayashi K, Asami Y, and Homma M. (2016) Domain-based biophysical characterization of the structural and thermal stability of FliG, an essential rotor component of the Na+-driven flagellar motor. Biophy. Physicobiol. 13: 227-233. doi: 10.2142/biophysico.13.0_227.
  • Takekawa N, Kwon S, Nishioka N, Kojima S, Homma M. (2016) HubP, a polar landmark protein, regulates flagellar number by assisting in the proper polar localization of FlhG in Vibrio alginolyticus. J Bacteriol. 198(22): 3091-3098. doi: 10.1128/JB.00462-16.
  • Takekawa N, Terahara N, Kato T, Gohara M, Mayanagi K, Hijikata A, Onoue Y, Kojima S, Shirai T, Namba K, Homma M. (2016) The tetrameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium. Sci Rep. 6: 31526. doi: 10.1038/srep31526.
  • Nishikino T, Zhu S, Takekawa N, Kojima S, Onoue Y, Homma M. (2016) Serine suppresses the motor function of a periplasmic PomB mutation in the Vibrio flagella stator. Genes Cells. doi: 10.1111/gtc.12357.
  • Tohru Minamino, Miki Kinoshita, Yumi Inoue, Yusuke V. Morimoto, Kunio Ihara, Satomi Koya, Noritaka Hara, Noriko Nishioka, Seiji Kojima, Michio Homma & Keiichi Namba. (2016) FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella. Microbiologyopen doi: 10.1002/mbo3.340.
  • Nishiyama S, Takahashi Y, Yamamoto K, Suzuki D, Itoh Y, Sumita K, Uchida Y, Homma M, Imada K, Kawagishi I. (2016) Identification of a Vibrio cholerae chemoreceptor that senses taurine and amino acids as attractants. Sci Rep. 6: 20866. doi: 10.1038/srep20866.
  • 2015Ishii E, Chiba S, Hashimoto N, Kojima S, Homma M, Ito K, Akiyama Y, Mori H. (2015) Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria. Proc Natl Acad Sci U S A. 112(40): E5513-22.
  • Masaru Kojima, Tatsuya Miyamoto, Masahiro Nakajima, Michio Homma, Tatsuo Arai, Toshio Fukuda. (2015) Bacterial sheet-powered rotation of a micro-object. Sensors and Actuators B: Chemical doi:10.1016/j.snb.2015.07.071.
  • Takekawa N, Nishiyama M, Kaneseki T, Kanai T, Atomi H, Kojima S, Homma M. (2015) Sodium-driven energy conversion for flagellar rotation of the earliest divergent hyperthermophilic bacterium. Sci Rep. 5: 12711. doi: 10.1038/srep12711.
  • Onoue Y, Kojima S, Homma M. (2015) Effect of FliG three-amino-acids deletion in Vibrio polar-flagellar rotation and formation. J Biochem. mvv068.
  • Ono H, Takashima A, Hirata H, Homma M, Kojima S. (2015) The MinD homolog FlhG regulates the synthesis of the single polar flagellum of Vibrio alginolyticus. Mol Microbiol. doi: 10.1111/mmi.13109.
  • Zhu S, Kumar A, Kojima S, Homma M. (2015) FliL associates with the stator to support torque generation of the sodium-driven polar flagellar motor of Vibrio. Mol Microbiol. doi: 10.1111/mmi.13103.
  • Nishino Y, Onoue Y, Kojima S, Homma M. (2015) Functional chimeras of flagellar stator proteins between E. coli MotB and Vibrio PomB at the periplasmic region in Vibrio or E. coli. Microbiologyopen. 4(2): 323-331.
  • Ogawa R, Abe-Yoshizumi R, Kishi T, Homma M and Kojima S. (2015) Interaction of the C-terminal tail of FliF with FliG from the Na+-driven flagellar motor of Vibrio alginolyticus. J Bacteriol. 197(1): 63-72.
  • 2014Hypoxia-induced localization of chemotaxis-related signaling proteins in Vibrio cholerae.